Cell Lines

hEC cell derived:

 

Endothelial Cells – Derived by FACS isolation based on a CD45- / VEGFR2 + cell surface profile. They have been evaluated for expression of PECAM, VE-Cadherin and VEGFR2.

 

Endothelial Cells – Notch ICD tet-on system and COUP-TFII tet-on system

 

Hepatoblasts – Derived from hEC exposed to liver-inducing conditions (Shiraki et al., 2008) and based on their ability to activate the HNF4 alpha promoter and a YFP reporter.

 

Dermal neonatal fibroblasts – isolated from foreskins.

Code

VNMRJ pulse sequence for flow mapping

 

SEMSFLOW3.c sequence to process flow data

 

Instructions on how to use the SEMSFLOW2 pulse sequence (Word & Text file formats):

http://bouchard.chem.ucla.edu/3D_neovascular_technology/semsflow2_readme.docx

http://bouchard.chem.ucla.edu/3D_neovascular_technology/semsflow2_readme.txt

 

Matlab program to process flow data from SEMSFLOW2.c sequence

 

Matlab program to process flow data from SEMSFLOW2.c sequence, also performs the flow/no flow subtraction

 

Matlab program to read FID files (works for SEMS sequence)

 

Matlab program to fit T2 maps with the MEMS sequence, monoexponential decay curves with baseline are fitted at each pixel

 

Matlab program to read multi-echo-multi-slice (MEMS) data sets

 

A useful Matlab colormap